When carbohydrates are fermented by bacteria, they produce acidic products. A change in pH can be detected when fermentation of a given carbohydrate has occurred. Acids lower the pH of the medium which will cause the pH indicator of Phenol Red to turn Yellow. When bacteria does not ferment the carbohydrate, the media remains red. Sometimes during fermentation, gas is produced. The Durham tube will then have a gas bubble trapped within it. There are three carbohydrate tests that can be performed. They are the Glucose (Dextrose) test, Lactose Test and the Sucrose Test. In all of these tests, the bacteria will be inoculated to the medium using a transfer loop. The results obtained will be similar to that illustrated in the picture below. The left tube shows less acid formation than the far right tube, but gas is still produced in this case. The Centre tube shows no carbohydrate utilization as there is neither any acid or gas produced. The medium remains the same. The Right tube shows acid is produced as evidenced by the yellow colouration; and gas is produced (an air bubble is trapped in the Durham tube).
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