Starch hydrolysis is a test used to detect the enzyme Amylase, which breaks down starch. After incubation, the plate is treated with Gram’s iodine. If the starch has been hydrolysed, then a reddish colour of clear zone will be formed around the bacterial growth. However, if the starch has not been hydrolysed, a blue or black area will be formed indicating the presence of starch. This test can be easily conducted by using an inoculating loop to spread bacteria on the plate surface. After it is incubated at an appropriate temperature, iodine will be added to the surface. Then, the colour change should be noted to know the results.
http://homepages.wmich.edu/~rossbach/bios312/LabProcedures/Starch%20Hydrolysis%20Medium.html
A. Absence of starch hydrolysis is indicated by the blue colour that completely surrounds the colony.
A. Absence of starch hydrolysis is indicated by the blue colour that completely surrounds the colony.
http://homepages.wmich.edu/~rossbach/bios312/LabProcedures/Starch%20Hydrolysis%20Medium.html
B. Starch hydrolysis is indicated by the reddish colour of clear zone.
B. Starch hydrolysis is indicated by the reddish colour of clear zone.
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