This is a test to detect the enzyme Urease which breaks down urea into ammonia. The liquid media is inoculated with a transfer loop. Since Ammonia is base, it will change the pH of the media. This change can be detected by using a pH indicator. In this case, the indicator being used is phenol red. Positive Results: The medium’s colour changes from yellow to bright pinkish-red; which indicates the presence of the enzyme Urease. Negative Results: The medium remains yellow.
http://www.bch.msu.edu/
www.bch.msu.edu
No comments:
Post a Comment