This tests for the ability of bacteria to convert Citrate, which is an intermediate of the Kreb’s Cycle into Oxaloacetate, which is another intermediate of the Kreb’s Cycle. In the media being used, Citrate is the only Carbon source available for usage for the survivial of the bacteria. If Citrate is not utilized, the bacteria will not grow. However, if the Citrate is utilized, the bactaria will grow on the media slant, and cause it to turn bright blue; as a result of the increase in pH. A transfer loop has to be utilized to transfer the bacteria onto the medium.
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