Indole Production
This is a test to determine if bacteria can breakdown amino acid tryptophan into Indole.
SIM media or TSB (Tryptic soy broth) is inoculated using a transfer loop. Kovac's reagent is added to media after 48 hours of incubation, to determine if Indole is produced by the bacteria. Positive Results: A Red layer forms on top of media (It signifies that the bacteria is able to break down the tryptophan to form Indole). Negative Results: No red layer will be formed.
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