Gelatine Utilization is used to test if bacteria can digest the Protein Gelatine. An enzyme called the Gelatinase must be produced by the bacteria to digest Gelatin. A transfer needle should be used to stab the gelatine, to inoculate the media. The inoculated media should be incubated for at least 48 hours. Then it should be transferred to the refrigerator. The media should be completely chilled before it is taken out for observation. If the media is solid after refrigeration it means that the result is negative. This means that the bacteria did not digest the gelatine. However, if the media is still in a liquid state after refrigeration, the test result is positive. This means that the bacteria has digested the gelatine.
The 'Serratia marcescens' on the left is positive for gelatinase production, as evidenced by the liquidation of the media. The 'Salmonella typhimurium' on the right is negative, as evidenced by the solidity of the media.
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