The oxidase test identifies organisms that produce the enzyme cytochrome oxidase by Gram- negative bacteria. Cytochrome oxidase involves in the electron transport chain by transferring electrons from a donor molecule to oxygen. It is a hallmark test for the Neiserria. It is also used to discriminate between aerobic Gram-negative organisms like Pseudomonas aeruginosa and other Enterobacteriaciae.
The test can be carried out simply by swabbing some of the test culture into one of the boxes on an oxidase dry slide. If there is a colour change of purple or blue at 30seconds – 1 minute; then the result is positive.
microbiology.scu.edu.tw/micromicrobe-exp/exp_website/Exp_30.htm
http://web.fccj.edu/~lnorman/unknowns.htm?index=2
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