This is a test to determine if glucose can be converted into acidic products. Bacteria is inoculated into a tube with a sterile transfer loop. The tube is incubated at 35°C between 2-5 days. After incubation, 2.5ml of the medium is transferred to another tube and five drops of the pH indicator methyl red is added to this tube. Thereafter, it is gently stirred, and any colour changes is noted down. Positive Results: Red colouration (Glucose is converted to acidic products). Negative Results: Yellow-orangish colour (Glucose is converted to neutral products)
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