Indole Production
This is a test to determine if bacteria can breakdown amino acid tryptophan into Indole.
SIM media or TSB (Tryptic soy broth) is inoculated using a transfer loop. Kovac's reagent is added to media after 48 hours of incubation, to determine if Indole is produced by the bacteria. Positive Results: A Red layer forms on top of media (It signifies that the bacteria is able to break down the tryptophan to form Indole). Negative Results: No red layer will be formed.
Tuesday, July 21, 2009
CITRATE UTILIZATION
Citrate Utilization:
This tests for the ability of bacteria to convert Citrate, which is an intermediate of the Kreb’s Cycle into Oxaloacetate, which is another intermediate of the Kreb’s Cycle. In the media being used, Citrate is the only Carbon source available for usage for the survivial of the bacteria. If Citrate is not utilized, the bacteria will not grow. However, if the Citrate is utilized, the bactaria will grow on the media slant, and cause it to turn bright blue; as a result of the increase in pH. A transfer loop has to be utilized to transfer the bacteria onto the medium.
This tests for the ability of bacteria to convert Citrate, which is an intermediate of the Kreb’s Cycle into Oxaloacetate, which is another intermediate of the Kreb’s Cycle. In the media being used, Citrate is the only Carbon source available for usage for the survivial of the bacteria. If Citrate is not utilized, the bacteria will not grow. However, if the Citrate is utilized, the bactaria will grow on the media slant, and cause it to turn bright blue; as a result of the increase in pH. A transfer loop has to be utilized to transfer the bacteria onto the medium.
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